Bioassay of cytokinin

Author: bhrz

August undefined, 2024

WebMar 1, 2009 · The development of sensitive analytical methods for the determination of cytokinin levels in plant tissues is essential for elucidating the roles of cytokinins in life science. In the past decade ... WebMay 1, 1972 · The cytokinin bioassay based upon these two effects is simple and provides results after a relatively short time. The root length is measured after 6 days of …

The biological activity of cytokinin derivatives in the soybean callus ...

WebCytokinin oxidase/dehydrogenase (CKO) is a flavoenzyme, which irreversibly degrades the plant hormones cytokinins and thereby participates in their homeostasis. ... all studied compounds were further analyzed for cytokinin activity in the Amaranthus bioassay and for binding to the Arabidopsis cytokinin receptors AHK3 and AHK4. By contrast to ... WebApr 28, 2006 · The classic cytokinin senescence bioassay that was modified to study abiotic stress responses was used in this study (Letham, 1971; Zwack et al., 2013Zwack et al., , 2016. Tomato leaf disks were ... show about giant flying dog

Cytokinins: Overview, Discovery, Types, Bioassay and …

WebThe activity, uptake and metabolism of cytokinin metabolites was determined in soybean (Glycine max (L.) Merr.) callus tissue. The following activity sequence was established: zeatin riboside (ZR)>zeatin (Z)>O-glucosides of Z, ZR and their dihydro derivatives>lupinic acid (an alanine conjugate of Z)>7- and 9-glucosides of Z which were almost inactive. Webyet rapid, bioassay for cytokinins (7). The sensitivity and specificity of several cytokinin bioassays, including those based on leaf senescence and callus formation, have been … WebThe induction of shoot buds from the filamentous protonema of moss is a classic bioassay for cytokinin. While a large literature documents this response in many species of moss … show about fox news

(PDF) A New Bioassay for Auxins and Cytokinins

Laboratory protocols for the quantitation of cytokines by …

WebNov 1, 2015 · Abstract. The cytokinins have been implicated in many facets of plant growth and development including cell division and differentiation, shoot and root growth, apical dominance, senescence, fruit and seed development, and the response to biotic and abiotic stressors. Cytokinin levels are regulated by a balance between biosynthesis [isopentenyl ... Web1-1. Introduction. We performed quantitative analysis for cytokinin using cotyledons (seed sprouts) of cucumber (Cucumis sativus L. cv.). We proposed to make E. coli produce cytokinin and needed to establish experimental system for quantitative analysis for cytokinin. Cucumber cotyledons are frequently used as samples for a simple and rapid … show about girl with powersWebHence, chlorophyll preservation test is a bioassay for cytokinin. Cytokinin promotes cell enlargement in the leaf tissues of certain plants. So, radish and bean leaf test have been used as bioassay. Promotion of lettuce seed germination forms the basis for another bioassay for cytokinins. Dwarf maize test is a bioassay for gibberellins as they ... show about george jones

"WebJul 23, 2024 · Cytokinin bioassay further demonstrated that S-4893 antagonized cytokinin-induced stimulation of callus formation and inhibition of root elongation. S … " - Bioassay of cytokinin

Bioassay of cytokinin

The stability and biological activity of cytokinin metabolites in ...

WebMay 1, 1972 · The cytokinin bioassay based upon these two effects is simple and provides results after a relatively short time. The root length is measured after 6 days of germination, while a-amylase activity is determined one day later in the same barley seedlings. The parallelism of these two effects indicates, better than a single test does, the presence ... WebXanthium expresses a large and rapid cytokinin response which can be obtained in very small solution volumes, and which is sensitive to cytokinin concen-trations as low as 10 …

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WebOct 2, 2016 · Prof. Bhagwan Deen. Physiological role: The cytokinin checks the degradation of chlorophyll and increases the RNA, DNA and protein content. It plays following role in plants: Cell division: Cytokinin promotes cell division in plant tissues in the presence of auxins. It promotes cell division in apical meristematic and cambium tissues … WebMay 15, 2013 · In a bioassay the activity of a sample is tested on a sensitive cell line and the results of this activity are compared to a standard cytokine preparation. This assay is …

WebThe synthetic cytokinin N6-cyclohexylmethyladenine exhibited inhibitory activity equivalent to that of 1-phenyl-3-(2-thiazolyl)-2-thiourea (PTTU), a compound known to be a potent inhibitor of dopamine-beta-hydroxylase activity. ... PTTU itself was found to exhibit cytokinin activity in the tobacco callus bioassay and to inhibit the activity of ... WebHence, chlorophyll preservation test is a bioassay for cytokinin. Cytokinin promotes cell enlargement in the leaf tissues of certain plants. So, radish and bean leaf test have been …

WebModifying the cytokinin content in plants is a means of improving plant productivity. Here, we report the development and biological activity of compound TD-K (1-(furan-2-ylmethyl)-3-(1,2,3-thiadiazol-5-yl)urea)which is related to thidiazuron. TD-K—which exhibited extremely high antisenescence activity in the wheat leaf bioassay—and INCYDE (2-chloro-6-(3 … WebThe test can measure cytokinin concentration between 0.001-10 mg/litre. It takes 3-5 weeks. 2. Retardation of Leaf Senescence: It is a rapid bioassay technique. Leaf discs …

WebJul 23, 2024 · Cytokinin bioassay further demonstrated that S-4893 antagonized cytokinin-induced stimulation of callus formation and inhibition of root elongation. S-4893 also promoted seminal, crown and lateral ...

WebFeb 1, 1998 · The purpose of these protocols is to provide practical stepwise methods for the bioassay of cytokines using cytokine responsive cell lines. They include tables of the … show about giving a kidneyWebThe bioassay is based on the expression in transformed tobacco ( Nicotiana tabacum) mesophyll protoplasts of a chimeric gene, consisting of the upstream sequences of the … show about god textingWebCytokinin is synthesized in the roots from where they are transported to shoots by xylem tissues. Biosynthesis of purine type of cytokinin is more clearly known than of other types. Various components of purine are derived from several small molecules including formyltetrahydro folate, aspartate, CO 2, ... show about girls stranded on islandWebJun 21, 2024 · 3. CYTOKININ • Cytokinins are a class of purine-type phytohormones that promote – cell division, – shoot and root morphogenesis, – chloroplast maturation, – cell … show about going back in time to dinosaursWebAbstract A rapid bioassay for cytokinins is described. This is based on the ability of these compounds to promote markedly the expansion of radish cotyledons excised soon after seed germination. ... naturally occurring substances without cytokinin activity, induce small growth increments in the assay. Cytokinin structure-activity relationships ... show about gold miningWebMar 1, 1974 · Cucumber cotyledon expanSIon in dark can therefore serve as a bioassay system for cytokinins. A comparison of the activity of different concentrations of kinetin and benzylaminopurine on cotyledon expansion in dark is shown in table 6 and figs. 3 to 5. It will be seen that benzylaminopurine is more active than kinetin in cucumber cotyledons ... show about haunted objectsWebSep 8, 2024 · Types of Cytokinins . There are two types of cytokinins: Adenine-type Cytokinins-Kinetin, Zeatin, and 6-benzyl amino purine … show about guys roasting